primary antibodies against nrf2  (Proteintech)

Journal: Materials Today Bio

Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

doi: 10.1016/j.mtbio.2026.102808

Figure Lengend Snippet: Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibody against Nrf2 (1:50, Abmart, #T55136) was added and incubated overnight at 4 °C, followed by rewarming at room temperature for 15 min and three washes with PBS.

Techniques: Immunofluorescence, Fluorescence, Staining, Western Blot, Biomarker Discovery, Expressing

Journal: Advanced Science

Article Title: Precision‐Engineered Silver Single‐Atom Carbon Dot Nanozymes for Theranostic Management of Acute Kidney Injury

doi: 10.1002/advs.202519393

Figure Lengend Snippet: Schematic illustration of the synthesis and therapeutic mechanism of mitochondria‐targeted T‐Ag SA ‐CDs for AKI therapy. In cisplatin‐induced AKI, T‐Ag SA ‐CDs (<10 nm) scavenge ROS via SOD‐ and GP X ‐like activities, restore mitochondrial membrane potential (Ψm), and activate the KEAP‐1/Nrf2 pathway, thereby upregulating HO‐1 and GPX4 to enhance antioxidant defense and alleviate renal injury.

Article Snippet: Primary antibodies against NRF2 (80593‐1‐RR), KEAP1 (80744‐1‐RR), HO‐1 (81281‐1‐RR), GPX4 (82822‐2‐RR), β‐actin (81115‐1‐RR), and NF‐κB (80979‐1‐RR), and HRP‐conjugated goat anti‐rabbit IgG secondary antibody (SA00001‐2), were obtained from Proteintech Group, Inc. Chemiluminescent detection was performed using the SuperKine West Pico PLUS substrate (BMU101‐CN, Abbkine Scientific Co., Ltd.).

Techniques: Membrane

Journal: Advanced Science

Article Title: Precision‐Engineered Silver Single‐Atom Carbon Dot Nanozymes for Theranostic Management of Acute Kidney Injury

doi: 10.1002/advs.202519393

Figure Lengend Snippet: Molecular and cellular responses to CDs based AKI response. a) Schematic representation of the cellular pathway involving KEAP1, NRF2, and downstream targets (HO‐1, GPX4) in response to AKI. b) Western blot analysis showing protein expression levels of KEAP1, NRF2, GPX4, HO‐1, and NF‐κB across experimental conditions (I: H 2 O 2 ; II: H 2 O 2 + N‐CDs; III: H 2 O 2 + Ag SA ‐CDs; IV: H 2 O 2 + T‐Ag SA ‐CDs and V: PBS) against β‐actin as a loading control. c) Ex vivo fluorescence imaging of N‐CDs, Ag SA ‐CDs, and T‐Ag SA ‐CDs at 1, 3, 6, 12, and 24 h, indicating time‐dependent changes in uptake and fluorescence intensity. d) Ex vivo biodistribution data of CDs in brain, heart, lung, spleen, liver, and kidney at 1, 2, 3, 6, 12, and 24 h, visualized through fluorescence imaging with a color scale representing signal intensity. e) Quantitative analysis of T‐Ag SA ‐CDs accumulation (% ID/g) in major organs (kidney, liver, lung, heart, brain, and spleen) time points of post‐injection ( n = 3 biologically independent mice). The i.v. dosage: 200 µL. Error bars represent standard deviations ( n = 3).

Article Snippet: Primary antibodies against NRF2 (80593‐1‐RR), KEAP1 (80744‐1‐RR), HO‐1 (81281‐1‐RR), GPX4 (82822‐2‐RR), β‐actin (81115‐1‐RR), and NF‐κB (80979‐1‐RR), and HRP‐conjugated goat anti‐rabbit IgG secondary antibody (SA00001‐2), were obtained from Proteintech Group, Inc. Chemiluminescent detection was performed using the SuperKine West Pico PLUS substrate (BMU101‐CN, Abbkine Scientific Co., Ltd.).

Techniques: Western Blot, Expressing, Control, Ex Vivo, Fluorescence, Imaging, Injection

Journal: Pharmaceutics

Article Title: Nobiletin Attenuates Inflammation and Modulates Lipid Metabolism in an In Vitro Model of Intestinal Failure-Associated Liver Disease

doi: 10.3390/pharmaceutics18010087

Figure Lengend Snippet: Effect of NOB, LPS, and INT on oxidative stress ( A ) and Nrf2 signaling pathways ( B ). ROS levels in THLE-2 cells after 24 h treatment with LPS, INT, INT+LPS, and INT+LPS+NOB (25 µM). Non-treated cells and DOXO (100 nM) served as negative and positive controls, respectively. ROS (+) and ROS (−) indicate cells with detectable or undetectable superoxide radicals, respectively. Representative histograms are shown, and data represent mean ± SEM from two independent experiments run in duplicate. Nrf2 subcellular distribution (cytosolic and nuclear fractions) and cytosolic SOD1 levels in THLE-2 cells after 24 h treatment. Representative blots are shown along with the corresponding Stain-Free™ total protein images used for total protein normalization (TPN). Lane order: (1) control, (2) LPS, (3) INT, (4) INT+LPS, (5) INT+LPS+NOB (10 µM), (6) INT+LPS+NOB (25 µM). Band intensities were quantified by densitometry and normalized to the lane-specific total protein signal obtained from Stain-Free imaging. Data are presented as mean ± SEM from two independent experiments, each performed in duplicate. Asterisks above denotes statistical significance relative to the untreated control cells with * p < 0.05, ** p < 0.01. Hashtags above denotes statistical significance relative to the INT+LPS-treated cells with # p < 0.05, ## p < 0.01, ### p < 0.001.

Article Snippet: Target proteins were detected using primary antibodies against Nrf2 and SOD1 (Santa Cruz Biotechnology, Dallas, TX, USA), followed by AP- or HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA and BosterBio, Pleasanton, CA, USA).

Techniques: Protein-Protein interactions, Staining, Control, Imaging

Journal: World Journal of Diabetes

Article Title: Effect of fish scale ointment on diabetic foot ulcer by inducing ferroptosis via the nuclear factor E2-related factor 2 pathway

doi: 10.4239/wjd.v16.i12.111789

Figure Lengend Snippet: Levels of ferroptosis-related proteins in rat wound tissues. A: Western blot bar plot of the proteins; B: Nuclear factor E2-related factor 2 protein expression level; C: Heme oxygenase-1 protein expression level; D: Glutathione peroxidase 4 protein expression level. a P < 0.05 vs control; b P < 0.05 vs model; c P < 0.05 vs fish scale collagen. GPX4: Glutathione peroxidase 4; HO-1: Heme oxygenase-1; Nrf2: Nuclear factor E2-related factor 2; FSC: Fish scale collagen; Lip-1: Liproxstatin-1.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-rat primary antibodies against Nrf2 (No. 12721, 1:1000), heme oxygenase-1 (HO-1) (No. 70081, 1:1000), glutathione peroxidase 4 (GPX4; No. 52455, 1:1000), and β-actin (No. 4967, 1:1000), all from Cell Signaling Technology, United States.

Techniques: Western Blot, Expressing, Control